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According to the Twelve O'Clock High’s prologue: “The air battle scenes in this Motion Picture were photographed in actual combat by members of the United States Air Force and the German Luftwaffe.”
Originally, this film was to be shot in Technicolor. Perhaps Darryl F. Zanuck’s lack of confidence in the public’s desire to see a war film can account for it being shot in black-and-white.
Clark Gable was interested in starring, and John Wayne passed on the lead.
The story was based on experiences of the U.S. Army Air Force during World War II. Due to the Pentagon's concerns over the script, the lead character's breakdown was softened.
Dean Jagger won an Academy Award for Best Supporting actor.
Anne Baxter and John Hodiak, who were married at the time, attended the world premiere in Los Angeles.